Wave Life Sciences Highlights Therapeutic Potential for WVE-006 for Alpha-1 Antitrypsin Deficiency and Progress Bringing RNA Editing to the Clinic During Analyst and Investor Event
WVE-006 is the most advanced candidate for AATD designed to restore functional wild-type AAT protein and reduce Z-AAT protein aggregation with potential for disease modification in both lung and liver phenotypes
IND-enabling activities for WVE-006 are underway and Wave expects to submit clinical trial applications for WVE-006 in 2023
Wave is also pioneering new therapeutic applications for RNA editing, including activating gene pathways and upregulating gene expression
“Wave continues to lead the genetic medicines field in advancing therapeutic RNA editing toward the clinic with our WVE-006 AIMer program and we expect to submit clinical trial applications for our first-in-human study in 2023. WVE-006 is distinct from other approaches in development for AATD, as it offers the opportunity to treat the root genetic cause of the disease and restore functional, wild-type protein that remains under physiological regulation. This would be a holistic solution and applicable to those with lung disease, liver disease, or both,” said
Highlights from today’s event, “Towards the Clinic: Spotlight on RNA Editing for AATD,” are below.
Best-in-Class Potential of WVE-006 for AATD
WVE-006 is a first-in-class, GalNAc-conjugated RNA editing candidate and the most advanced program currently in development using an oligonucleotide to harness an endogenous enzyme for editing. WVE-006 is designed to correct the single G-to-A base mutation in mRNA coded by the SERPINA1 Z allele, thereby enabling functional, wild-type M-AAT protein to be produced.
Data for WVE-006 highlighted today include:
- At 13 weeks in an AATD mouse model (NSG-PiZ), treatment with WVE-006 resulted in approximately 50% RNA editing of SERPINA1 transcript. Additionally in this same preclinical study:
- AAT protein levels in mice dosed with WVE-006 were approximately 7-fold greater than
PBS-administered controls and well above the established protective threshold of 11 uM.
- WVE-006 led to restoration of approximately 50% wild-type M-AAT protein in serum.
- WVE-006 led to a 3-fold increase in neutrophil elastase inhibition activity, indicating the restored M-AAT protein was functional.
- AAT protein levels in mice dosed with WVE-006 were approximately 7-fold greater than
- An earlier lead (pre-optimization) AIMer reduced Z-AAT aggregates and inflammation in mouse livers (mouse model: huADARxSA1).
- Wave’s AATD AIMers are highly specific in vitro and in vivo based on transcriptome-wide analyses.
WVE-006 Advancing Toward the Clinic
IND-enabling activities for WVE-006 are underway and Wave expects to submit clinical trial applications (CTAs) for WVE-006 in 2023.
In addition, Wave announced today initial plans for the anticipated first-in-human study of WVE-006. This Phase 1/2 study would evaluate safety, pharmacokinetics, and relevant biomarkers, including serum AAT. The study, which would use Wave’s adaptive design approach, is expected to first enroll healthy volunteers to establish dose, followed by cohort expansion with Pi*ZZ patients to evaluate target engagement based on change in serum AAT levels.
Future Applications of AIMers
Wave is advancing research using AIMers for applications beyond correction of G-to-A driver mutations and today highlighted preclinical data supporting the use of AIMers to activate gene pathways and upregulate gene expression. These applications would significantly expand the universe of targets addressable with AIMers, including diseases with large patient populations. Highlights from the presentation include:
- GalNAc-AIMers can disrupt KEAP1 protein/NRF2 protein interactions, thereby enabling NRF2 downstream gene upregulation in the liver of mice.
- GalNAc-AIMers can edit RNA motifs to restore or upregulate gene expression in the liver of mice.
Wave is also advancing research using AIMers beyond hepatocytes and using free uptake instead of GalNAc conjugation. Preclinical in vivo data supports the ability to edit with systemic delivery in multiple tissues, including heart, kidney, lung, spleen, liver, white adipose tissue, and brown adipose tissue. Wave has also generated in vivo data demonstrating substantial and durable editing and supporting administration of AIMers with intrathecal or intracerebroventricular delivery in central nervous system tissues.
“Towards the Clinic: Spotlight on RNA Editing for AATD” Presentation and Slideshow
A replay of today’s webcast and the full slideshow presentation can be accessed and downloaded by visiting “Events & Publications” on the investor relations section of the
Wave’s AIMers are designed to correct mutations in an RNA transcript, thereby avoiding permanent changes to the genome that occur with DNA-targeting approaches. Rather than using an exogenous editing enzyme, AIMers recruit proteins that exist in the body, called ADAR enzymes, which naturally edit certain adenine (A) bases to inosine (I). Because I is read as G (guanine) by the cellular translational machinery, sequence-directed editing with ADAR has the potential to revert transcripts with single G-to-A point mutations that cause genetic diseases. This approach redirects a natural system for therapeutic purposes, enables simplified delivery without viral particles or liposomes, and avoids the risk of irreversible off-target effects of DNA-targeting approaches. AIMers are short in length, fully chemically modified, and use novel chemistry, including proprietary PN backbone modifications and chiral control, that make them distinct from other ADAR-mediated editing approaches.
About Alpha-1 antitrypsin deficiency (AATD)
Alpha-1 antitrypsin deficiency (AATD) is an inherited genetic disorder that is commonly caused by a G-to-A point mutation (“Z allele”) in the SERPINA1 gene. This mutation leads to lung disease due to lack of wild-type alpha-1 antitrypsin (M-AAT) in lungs and liver disease due to aggregation of misfolded Z-AAT protein in hepatocytes. There are approximately 200,000 patients in
PRISM is Wave Life Sciences’ proprietary discovery and drug development platform that enables genetically defined diseases to be targeted with stereopure oligonucleotides across multiple therapeutic modalities, including silencing, splicing, and editing. PRISM combines the company’s unique ability to construct stereopure oligonucleotides with a deep understanding of how the interplay among oligonucleotide sequence, chemistry and backbone stereochemistry impacts key pharmacological properties. By exploring these interactions through iterative analysis of in vitro and in vivo outcomes and machine learning-driven predictive modeling, the company continues to define design principles that are deployed across programs to rapidly develop and manufacture clinical candidates that meet pre-defined product profiles.
This press release contains forward-looking statements within the meaning of the Private Securities Litigation Reform Act of 1995, as amended, including, without limitation, our expectations for our GalNAc-conjugated A-to-I(G) RNA base editing oligonucleotides (AIMers) and the anticipated therapeutic benefits thereof; our belief that WVE-006 is the most advanced candidate for AATD designed to restore functional wild-type AAT protein and reduce Z-AAT protein aggregation; our expectations regarding the ability of our AIMers to address diseases of many different tissues and cell types; the potential benefits of our AIMers compared with other RNA base editing approaches; the anticipated timing of future development milestones for our lead AIMer program, WVE-006; and our expectations regarding potential future applications of our AIMERs to activate genetic pathways and upregulate gene expression. The words “may,” “will,” “could,” “would,” “should,” “expect,” “plan,” “anticipate,” “intend,” “believe,” “estimate,” “predict,” “project,” “potential,” “continue,” “target” and similar expressions are intended to identify forward-looking statements, although not all forward-looking statements contain these identifying words. Any forward-looking statements in this press release are based on management's current expectations and beliefs and are subject to a number of risks, uncertainties and important factors that may cause actual events or results to differ materially from those expressed or implied by any forward-looking statements contained in this press release and actual results may differ materially from those indicated by these forward-looking statements as a result of these risks, uncertainties and important factors, including, without limitation, the risks and uncertainties described in the section entitled “Risk Factors” in Wave’s most recent Annual Report on Form 10-K filed with the
Source: Wave Life Sciences USA, Inc.